Human Coronary Artery Endothelial Cells (HCAECs)

Product Description

Human Coronary Artery Endothelial Cells (HCAEC) line the vessel wall of coronary artery. They are extremely important in regulation of coronary blood flow and cardiac function. HCAEC has been used as a valuable in vitro model system to study the cardiovascular diseases such as thrombosis, atherosclerosis, hypertension, coronary artery diseases ^[1] and diabetes-related cardiovascular diseases ^[2].

iXCells Biotechnologies provides high quality HCAEC, which are isolated from human coronary artery from single donors and cryopreserved at P2, with >0.5 million cells in each vial. These HCAEC express vWF/Factor VIII and CD31 (Figure 1). They are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi and can further expand in Endothelial Cell Medium (Cat# MD-0010) under the condition suggested by iXCells Biotechnologies.

Figure 1.  HCAECs provided by iXCells are positive for CD31 and vWF, as shown by immunostaining (A and B) and flow analysis (A' and B').  More than 97% of cells are positive for both markers by flow analysis (A' and B'). 

Product Details

References

[1]  Besler C,et al and Landmesser U. Mechanisms underlying adverse effects of HDL on eNOS-activating pathways in patients with coronary artery disease. J Clin Invest. 2011; 121 (7): 2693-2708.

[2]  Eriksson L and Nystrom T. Activation of AMP-activated protein kinase by metformin protects human coronary artery endothelial cells against diabetic lipoapoptosis. Cardiovasc Diabetol. 2014; 13:152

 Download Datasheet