Rat Hepatic Stellate Cells (RHSC)

Product Description

Hepatic stellate cells (HSC) are pericytes found in the perisinusoidal space presenting myofibroblast-like or lipocyte phenotypes. They participate in the homeostasis, repair, and regeneration of liver extracellular matrix, and control retinol metabolism, storage, and release. Following liver injury, HSC proliferate, transform into myofibroblast-like cells, and produce type I collagen in the fibrotic liver. HSC have been implicated as a regulator of hepatic microcirculation via cell contraction in the pathogenesis of intrahepatic portal hypertension [1]. HSC also possess voltage-activated calcium current, express the low affinity nerve growth factor receptor p75, and undergo apoptosis in response to nerve growth factor stimulation [2, 3]. These insights into the molecular regulation of HSC activation will help lead to new therapeutic approaches in treatment of hepatic fibrosis by reducing morbidity and mortality in patients with chronic liver injury.

iXCells Biotechnologies provides high quality Rat Hepatic Stellate Cells (RHSC), which are isolated from young DS rat liver and cryopreserved at P2, with >0.5 million cells in each vial. RHSC express desmin and α-actin. They are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi and can further expand for 5 population doublings in Stellate Cell Growth Medium (Cat# MD-0014) under the condition suggested by iXCells Biotechnologies.

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References

[1] Reynaert H, Thompson MG, Thomas T, Geerts A. (2002) Hepatic stellate cells: role in microcirculation and pathophysiology of portal hypertension. Gut 50:571-581. 
[2] Rockey D. C. (2001) Hepatic blood flow regulation by stellate cells in normal and injured liver. Semin Liver Dis 21(3):337-49.
[3] Oide H, Tateyama M, Wang XE, Hirose M, Itatsu T, Watanabe S, Ochi R, Sato N. (1999) Activated stellate (Ito) cells possess voltage-activated calcium current. Biochim. Biophys. Acta. 1418:158-164. 
[4] Trim N, Morgan S, Evans M, Issa R, Fine D, Afford S, Wilkins B, Iredale J. (2000) Hepatic stellate cells express the low affinity nerve growth factor receptor p75 and undergo apoptosis in response to nerve growth factor stimulation. Am. J. Pathol. 156(4):1235-1243.

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