Mouse Hepatic Stellate Cells (MHSC)

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Product Description

Hepatic stellate cells (HSCs) are liver-specific mesenchymal cells, and account for 5~8% of the cells in the liver. HSCs play vital roles in the homeostasis of liver extracellular matrix, repair, regeneration and fibrosis, and control retinol metabolism, storage and release. The stellate cell is the major cell type involved in liver fibrosis in response to liver injury [1]. In healthy liver, HSCs are in a quiescent state, and contains numerous vitamin A lipid droplets, constituting the largest reservoir of vitamin A in the body. When the liver is damaged, HSCs can change into an activated state, which is characterized by proliferation, contractility and chemotaxis. The amount of vitamin A decreases progressively in injured liver. The activated HSCs also secrete collagen scar tissue, which can lead to cirrhosis. In chronic liver disease, prolonged and repeated activation of stellate cells causes liver fibrosis [2].

Primary culture of HSC is a valuable tool to study liver fibrosis. iXCells Biotechnologies provides high quality frozen or freshly plated mouse HSCs (MHSC) isolated from mouse liver. The cells are frozen immediately after isolation, with >0.5 million cells per vial, or shipped one day after plating. There will be 30-50% dead cells after thawing, which is normal for MHSC.

Figure 1. (A) Phase contrast image of day 3 culture of mouse hepatic stellate cells (MHSC).  (B). Vitamin A droplets in MHSC were visualized by blue autofluorescence (lower panel) when excited with UV light.

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